02043nas a2200217 4500008004100000245011700041210006900158490000800227520137600235653001701611653002001628653001801648653001001666653001401676653001301690653001401703100001801717700002101735700001901756856005001775 2006 eng d00aEvolutionary history and patterns of differentiation among European Maniola butterflies (Lepidoptera: Satyrinae)0 aEvolutionary history and patterns of differentiation among Europ0 v1033 a

Phylogenetic relationships of European Maniola butterflies are reconstructed using molecular sequences from two regions
of the mitochondrial DNA, cytochrome oxidase subunit I (COI) and cytochrome b (Cytb). A total of 988 base pairs (486 for Cytb,
and 502 for COI) were aligned for 15 individuals of Maniola and an outgroup species. The phylogenetic tree obtained through
Bayesian inference analysis of the combined data sets shows evidence that the island endemic M. chia is indistinguishable from M.
jurtina on the basis of the mtDNA genes studied. Net nucleotide divergence between M. jurtina and M. chia is 0.4%, but 2%
between the M. jurtina and the M. nurag clade. A phenetically distinct entity of individuals from Sardinia appears to be a hybrid
between M. nurag and M. jurtina. The southern and northern European ecotypes of M. jurtina, which differ in the summer aestivation
period of the southern type, are not structured genetically at the level of coding mtDNA genes. Divergence time between M.
nurag and M. jurtina was estimated to be 1.1 to 1.2 million years. Speciation most likely took place in the early Pleistocene as a consequence
of the isolation of Sardinia, when the sea reflooded the Mediterranean basin after the Messinian crisis (about 5 million
years ago).

10aManiola chia10aManiola jurtina10aManiola nurag10amtDNA10aphylogeny10aSardinia10aSatyrinae1 aGrill, Andrea1 aGkiokia, Erasmia1 aAlvarez, Nadir uhttps://lepidopteragr.myspecies.info/node/701