03386nas a2200289 4500008004100000245013000041210006900171490000700240520249000247653001602737653001702753653001502770653000802785653001702793653002002810653001602830653000902846653001402855653002502869653003402894653002202928653001302950100002802963700002802991700002703019856005003046 2016 eng d00aKaryosystematics and molecular taxonomy of the anomalous blue butterflies (Lepidoptera, Lycaenidae) from the Balkan Peninsula0 aKaryosystematics and molecular taxonomy of the anomalous blue bu0 v103 a

The Balkan Peninsula represents one of the hottest biodiversity spots in Europe. However, the invertebrate
fauna of this region is still insufficiently investigated, even in respect of such well-studied organisms as
Lepidoptera. Here we use a combination of chromosomal, molecular and morphological markers to rearrange
the group of so-called anomalous blue butterflies (also known as ‘brown complex’ of the subgenus
Agrodiaetus Hübner, [1822] and as the Polyommatus (Agrodiaetus) admetus (Esper, 1783) species group)
and to reveal its cryptic taxonomic structure. We demonstrate that P. aroaniensis (Brown, 1976) is not as
widespread in the Balkans as was previously thought. In fact, it has a dot-like distribution range restricted
to the Peloponnese Peninsula in South Greece. Polyommatus orphicus Kolev, 2005 is not as closely related
to the Turkish species P. dantchenkoi (Lukhtanov & Wiemers, 2003) as was supposed earlier. Instead, it
is a Balkan endemic represented by two subspecies: P. orphicus orphicus (Bulgaria) and P. orphicus eleniae
Coutsis & De Prins, 2005 (Northern Greece). Polyommatus ripartii (Freyer, 1830) is represented in the
Balkans by an endemic subspecies P. ripartii pelopi. The traditionally recognized P. admetus (Esper, 1783) is
shown to be a heterogeneous complex and is divided into Polyommatus admetus sensu stricto (the Balkans
and west Turkey) and P. yeranyani (Dantchenko & Lukhtanov, 2005) (east Turkey, Armenia, Azerbaijan
and Iran). Polyommatus nephohiptamenos (Brown & Coutsis, 1978) is confirmed to be a species with a dotlike
distribution range in Northern Greece. Finally, from Central Greece (Timfristos and Parnassos moun-tains) we describe Polyommatus timfristos Lukhtanov, Vishnevskaya & Shapoval, sp. n. which differs by its

haploid chromosome number (n=38) from the closely related and morphologically similar P. aroaniensis
(n=47-48) and P. orphicus (n=41-42). We provide chromosomal evidence for three separate south Balkan
Pleistocene refugia (Peloponnesse, Central Greece and Northern Greece/South Bulgaria) and stress the
biogeographic importance of Central Greece as a place of diversification. Then we argue that the data
obtained have direct implications for butterfly karyology, taxonomy, biogeography and conservation.

10aAgrodiaetus10abiodiversity10achromosome10aCOI10aconservation10acryptic species10aDNA barcode10aITS210akaryotype10amitochondrial marker10aPolyommatus timfristos sp. n.10aprotected species10ared list1 aVishnevskaya, Maria, S.1 aLukhtanov, Vladimir, A.1 aSaifitdinova, Alsu, F. uhttps://lepidopteragr.myspecies.info/node/70401481nas a2200205 4500008004100000245013700041210006900178490000700247520081500254653001601069653001601085653001101101653001601112653001501128653002101143653001701164100002001181700002401201856005001225 2015 eng d00aTaxonomic Position and Status of Polyommatus (Agrodiaetus) iphigenia (Lepidoptera, Lycaenidae) from the Peloponnese, Southern Greece0 aTaxonomic Position and Status of Polyommatus Agrodiaetus iphigen0 v633 a

In our study we use a 690 bp fragment of the COI gene to analyze a taxon from southern
Greece, usually treated as Polyommatus (Agrodiaetus) iphigenia nonacriensis (Brown,
1977). The previous conclusions on taxonomy and nomenclature of P. (A.) iphigenia
nonacriensis were not supported by molecular or cytological data, therefore the problem of
identity of this taxon has remained unsolved. We found that with respect to COI haplotypes,
P. (A.) iphigenia nonacriensis from Greece is similar to the studied populations of P. (A.)
iphigenia (Herrich-Schäffer, 1847) from Turkey and Armenia. Thus, we confirm that the
only Greek Agrodiaetus butterfly with blue wing coloration in males actually belongs to the
species P. (A.) iphigenia.

10aAgrodiaetus10abutterflies10aGreece10aLepidoptera10aLycaenidae10amolecular marker10aPeloponnese.1 aShapoval, Nazar1 aLukhtanov, Vladimir uhttps://lepidopteragr.myspecies.info/node/70303346nas a2200289 4500008004100000245013000041210006900171490000700240520245000247653001602697653001702713653001502730653000802745653001702753653002002770653001602790653000902806653001402815653002502829653003402854653002202888653001302910100002802923700002702951700002802978856005003006 2016 eng d00aKaryosystematics and molecular taxonomy of the anomalous blue butterflies (Lepidoptera, Lycaenidae) from the Balkan Peninsula0 aKaryosystematics and molecular taxonomy of the anomalous blue bu0 v103 a

The Balkan Peninsula represents one of the hottest biodiversity spots in Europe. However, the invertebrate
fauna of this region is still insufficiently investigated, even in respect of such well-studied organisms as
Lepidoptera. Here we use a combination of chromosomal, molecular and morphological markers to rearrange
the group of so-called anomalous blue butterflies (also known as ‘brown complex’ of the subgenus
Agrodiaetus Hübner, [1822] and as the Polyommatus (Agrodiaetus) admetus (Esper, 1783) species group)
and to reveal its cryptic taxonomic structure. We demonstrate that P. aroaniensis (Brown, 1976) is not as
widespread in the Balkans as was previously thought. In fact, it has a dot-like distribution range restricted
to the Peloponnese Peninsula in South Greece. Polyommatus orphicus Kolev, 2005 is not as closely related
to the Turkish species P. dantchenkoi (Lukhtanov & Wiemers, 2003) as was supposed earlier. Instead, it
is a Balkan endemic represented by two subspecies: P. orphicus orphicus (Bulgaria) and P. orphicus eleniae
Coutsis & De Prins, 2005 (Northern Greece). Polyommatus ripartii (Freyer, 1830) is represented in the
Balkans by an endemic subspecies P. ripartii pelopi. The traditionally recognized P. admetus (Esper, 1783) is
shown to be a heterogeneous complex and is divided into Polyommatus admetus sensu stricto (the Balkans
and west Turkey) and P. yeranyani (Dantchenko & Lukhtanov, 2005) (east Turkey, Armenia, Azerbaijan
and Iran). Polyommatus nephohiptamenos (Brown & Coutsis, 1978) is confirmed to be a species with a dotlike
distribution range in Northern Greece. Finally, from Central Greece (Timfristos and Parnassos moun-tains) we describe Polyommatus timfristos Lukhtanov, Vishnevskaya & Shapoval, sp. n. which differs by its haploid chromosome number (n=38) from the closely related and morphologically similar P. aroaniensis (n=47-48) and P. orphicus (n=41-42). We provide chromosomal evidence for three separate south Balkan Pleistocene refugia (Peloponnesse, Central Greece and Northern Greece/South Bulgaria) and stress the biogeographic importance of Central Greece as a place of diversification. Then we argue that the data obtained have direct implications for butterfly karyology, taxonomy, biogeography and conservation.

10aAgrodiaetus10abiodiversity10achromosome10aCOI10aconservation10acryptic species10aDNA barcode10aITS210akaryotype10amitochondrial marker10aPolyommatus timfristos sp. n.10aprotected species10ared list1 aVishnevskaya, Maria, S.1 aSaifitdinova, Alsu, F.1 aLukhtanov, Vladimir, A. uhttps://lepidopteragr.myspecies.info/node/69400507nas a2200121 4500008004500000245018100045210006900226300001200295490000700307653001600314100000500330856005000335 2007 Engldsh 00aNew data on the ecology of 8 taxa of Agrodiaetus H?bner, 1822 from Greece and Spain: hostplants, associated ants and parasitoids (Lepidoptera: Lycaenidae. Hymenoptera. Diptera)0 aNew data on the ecology of 8 taxa of Agrodiaetus Hbner 1822 from a189-1980 v3810aAgrodiaetus1 a uhttps://lepidopteragr.myspecies.info/node/47800449nas a2200121 4500008004500000245010900045210006900154300001200223490000600235653001600241100002000257856005000277 1986 Engldsh 00aThe blue butterflies of the genus Agrodiaetus H?bner (Lep.: Lycaenidae): Symptoms of taxonomic confusion0 ablue butterflies of the genus Agrodiaetus Hbner Lep Lycaenidae S a159-1690 v910aAgrodiaetus1 aG., Coutsis, J. uhttps://lepidopteragr.myspecies.info/node/289